Monday, March 6, 2017
MSQRD 1 0 3 APK
MSQRD 1 0 3 APK
MSQRD is a recently launched smartphone application from Masquerade Technologies which allows you to record and customize your photos and selfie video animations with a wide variety of unique looking features and send it to friends via your favorite messengers and social networks. The app is now available on Android and already to download on the Google Play Store. As always, you can download MSQRD apk here to install it directly on your Android device.

The aim of the app lets you create a terrifying videos that will forever haunt both your dreams and everyone elses. You take your short video and after recording, you will be sent to a menu where they will be given near endless options to add something silly to your selfie videos. MSQRD is an incredibly fun app that offers life filters for selfie photos and videos. From putting animal faces on yourself, to giving yourself a morbid makeover to wanting to look like a random celebrity.
The MSQRD app can give anyones selfie a creative, but overall hilarious, look. There are a wide selection of masks to choose from, ranging from cutesy stuff like bunny ears cat ears, cartoon eyes to animal masks featuring gorilla, polar bear and tiger faces, if youre feeling animalistic. There is also a selection of celebrity filters that allows you to take on the appearance of Harry Potter, Snoop Dogg, Leonardo DiCaprio, The Joker and Donald Trump to name a few.
See also:
Photo Selfie App
- Retrica
- Camera 360 Ultimate
Video Selfie App
- Dubsmash
Download MSQRD APK
File Name: msqrd-1.0.3.apkApp Name: MSQRD
Version: 1.0.3
File Size: 28 MB
Updated: March 6, 2016

The MSQRD APK file uploaded above is the original file extracted from Google Play Store in no way has been modified or altered. You can download this file without any hesitation.
Available link for download
Sunday, March 5, 2017
More New Rob Interviews From LIFE Promo ELLE Italy 3 Sat Online Szene Hamburg
More New Rob Interviews From LIFE Promo ELLE Italy 3 Sat Online Szene Hamburg
A couple more new translated interviews (some of the interviews look the same, but there are new questions, quotes...) and a new snippet from a video interview.
Click on the picture or link to watch

(Via | Translation)
A talk with Robert Pattinson
During the Berlinale we met the actor, who talked about his new movie LIFE, bad fathers, and photography as art and frost bite on fingers.
What was it like to play a photographer under the direction of a legendary photographer like Anton Corbijn?
(laughs) Luckily I didnt really see Dennis Stock as a photographer in the beginning. For me he was someone who wanted to be an artist but wasnt sure if he had what it takes to be an artist. I had the feeling that the camera was a means for him to express himself.
How did you work with the camera? Did you only pose with it or did you really use it?
The great thing was that I got the camera a couple of months before we started shooting. It was loaned to us from the Leica museum and its the same camera Dennis Stock used. There arent many original old cameras left, but they are great and I used mine extensively.
What did you photograph?
I started doing the Werner Herzog film Queen of the Desert right after that and I couldnt stop taking pictures of the location. I took hundreds of pics of the sets and Marrakesh. I didnt really do it seriously or because of the movie, but more because it was fun. I figured I could ask Anton how to use the Leica correctly later, but he couldnt really help me with that (laughs)
But he did show you some tricks, didnt he? I mean he has to be the best teacher for that.
Thats what I thought, that he would show me how to hold the camera and move it, but Anton told me that I needed to understand the camera myself. Eventually I understood him because he is a different photographer than Dennis Stock was. Anton loves photography, he likes moving on the sidelines and observe everything as oppose to Dennis Stock, who acted more like a painter. Stock wasnt only focused on his counterpart but also on himself and he was looking for validation. He didnt really enjoy his role as a photographer because he wanted to be more extravagant.
Is that the reason you were interested in the role?
To be honest the first thing that interested me was that he was a bad father. Usually at my age there arent many father roles to play and in this one the father doesnt love his kid and doesnt understand why. There is this beautiful scene where James Dean is playing with his nephew and Stock is watching them and wracking his brains how Dean can be so natural and loving with a child. That broke my heart. Another thing is, that everybody thinks that someone like that is an asshole and I thought it was exciting to present him more likable despite of that. Contrary to that Dennis Stocks pictures are full of feeling.
You can really see that, in his own way, he really loved James Dean. He couldnt really tell him that but it seems like Stock put a crown on James with the pictures. At the same time bitterness and jealousy also shine through those pictures and one could also see the influence James had on him. I love Stocks pictures from that era, the jazz musicians whose pictures showed how much he admired them. I think photography was a way for him to show his love for others.
Did the role change your view of photographers that follow you all the time?
Not really. Even if a photographer wanted to be paparazzi back then, it wouldnt have been easy without a lot of knowledge and skills, especially trying to use the flash (laughs) Apart from that people like Dennis Stock had a different aspiration to photography. They were searching for a new imagery and they wanted to present people in a different light. They want them to vibrate and discover new sides of them and the readers wanted that as well. Today one doesnt really need to do a lot to push the release and paparazzi are kind of trying to humiliate people. Its as if they dont really like what they are doing themselves and so they look for the bad things in others. I dont really understand it and its annoying.
Is that really Anton Corbijn standing on the red carpet playing a photographer in the movie?
Yes, thats him and he used more takes for his scene than any other in the movie (laughs) He would say Oh I didnt do that right and we shot until 10 am. It was crazy.
Is James Dean still important for young actors today? Is he still a role model?
I still remember when I was 16 and he was one of my idols. Everyone knows the picture on Times Square and he was the ideal model of understatement. When I started acting I was very timid and I didnt want to overact. I wanted my acting to be more like his: calm and full of feelings that he was able to internalize.
Was there a James Dean scene for you in the movie?
Yes, especially where the staying calm is concerned (laughs) we shot the scenes on Deans farm in Toronto and it was -40 degrees and I really couldnt understand how one can shoot outside in that cold. The camera froze to my fingers and I had to stand in front of a heater to get it off my fingers. We were really close to getting frostbite and I started to get annoyed, but then I was over that and I got really calm.
(Via | Translation)
Robert Pattinson is back in cinemas on October 8 with the film Life , an Anton Corbijns movie dedicated to James Dean, movie legend who died exactly 60 years ago in a car accident . The actor who became famous as a vampire in Twilight , however, did not take the role of the legendary Jimmy (his shoes are filled by Dane DeHaan ), as widely expected, but those of photographer Dennis Stock , who in 1955 that same year of the death the star snapped a series of photographs for the magazine Life immortalizing James Dean as no one before him had done. Anton Corbijn is dedicating the whole movie to the trusting relationship that comes to life between the two: to speak in more detail of the film is own Robert Pattinson , in this exclusive interview given during the filming of the movie.
Dennis and James. A photographer on the brink of bankruptcy and an artist on the crest of a wave. Tell us about the dynamics of their friendship.
The story of these two has a very original dynamics. James Dean is a character so emphatic and Dennis, is not. Theres a moment that, for me, sums up this diversity. Theres this scene where James Dean is playing with his nephew, and Dennis just says, I do not know how you can do it. Basically he is saying I do not understand what theyre feeling. Dennis had a son, of course, but he does not love him and thats just awful. He is constantly permeated by negativity, so full of anxiety, to the point that he becomes really irritating. You can hardly believe that these kinds of people exist, you cant believe the fact that they say they are not able to love. Hes kind of a horrible being, but in his tragedy hes also an extremely fascinating character. And as I said the dynamic between them is really interesting.
James Dean is a myth. Has he ever had an influence on your career?
I have long admired his work. I believe that a lot of 16 year old actors have had their James Dean stage and for most of them, the important thing is not the role to play but being part of the fantasies derived from the myth and I also have experienced one of the two phases. He is certainly still an icon but Dane (DeHaan) would be able to answer that question better than me because he is more tied to James Dean, his figure, the myth.
Would you have been interested in playing the part of James Dean?
Oh no, absolutely not (laughs) Dane did a great job.
How did it go with Anton Corbijn?
Working with Anton Corbijn has been a great honor and his first film Control (about the life of Ian Curtis, leader of Joy Division), was the reason why I decided just to accept the part. I loved that movie. I love the style of Anton and I knew that LIFE would have followed the same path.
And with the camera?
To practice at best, I took some pictures on the set of LIFE and of the other film that I was shooting The Queen of the Desert where I worked with Nicole Kidman. Then for a few months I took a number of wonderful, horrifying pictures with a Leica M1 of 1953. It wasnt the personal one used by Dennis, but the same model. It must have been a model that came out some time before Stocks. Its beautiful, and it works perfectly. I think that will never break.
Who was Dennis Stock?
Dennis was always worried that everything could go wrong. He felt haunted by the possibility that the public would not follow him, that they wouldnt be on his side. But at the same time, I thought he was a modern, contemporary character. The story is about someone trying to become an artist and the fear of not being able to achieve his dream is the saddest and demoralizing part of his life. Dennis is the kind of artist who is so afraid of not being good enough for his profession that he would use excuses for anything. When he sees Jimmy for the first time, its fun because he has precisely this undeniable effect. Being close to someone who is reaching their potential is a very nice process to witness. Relating to James Dean and everything that was happening to him allowed Dennis to believe a little more in himself too.
Do you believe then that James Dean had a positive influence on the photographer?
Absolutely yes. Sometimes you just need a little encouragement and the fact that Jimmy told him These are great while he showed him the pictures he had taken of him was a huge source of pride. I think at that specific time Dean clearly showed and paved the way for Stock. He regarded Jimmy as a true artist who had a profound impact on his life. And so, when Jimmy gave his approval, well, thats all you need sometimes this is all it takes to start believing in yourself. And I think thats what happened. For Dennis, the encounter with Jimmy was fundamental and has changed his life and certainly his work.
Available link for download
Saturday, March 4, 2017
N O V A 3 Freedom Edition v1 0 1d APK Mod Unlimited Money Android Games
N O V A 3 Freedom Edition v1 0 1d APK Mod Unlimited Money Android Games

The most immersive and impressive sci-fi FPS franchise on smartphones is now completely free! Fight for humankinds survival!
Four months after Kal Wardin defeated the Volterites, President Folsom is assassinated and the beleaguered colonies surrender to the Volterite Protectorate.
Since then, Kal has been lying low, but a desperate plea from Yelena calls him back to save Earth and humankind
An epic storyline: Humanity finally returns to Earth after years of exile! Fight in 10 immersive levels across the galaxy, from a war-torn Earth to a frozen Volterite city.
Multiple weapons and powers: Run, shoot, drive vehicles, and pilot a mech to defeat hordes of enemies.
Join 12-player battles in 7 multiplayer modes (Capture the Point, Free-for-All, Capture the Flag, etc.) on 7 different maps.
For the first time, multiple allies can jump inside the same vehicle and spread destruction on the battlefield.
- Install apk
- Extract rar file using winzip or unrar
- Put data folder com.gameloft.android.ANMP.GloftNAHM on Sdcardandroidobbhere
- Play the game
MOD
MOD
Available link for download
Friday, March 3, 2017
Motu And His Samosas Motu Patlu Compilation Part 3 50 Minutes of Fun!
Motu And His Samosas Motu Patlu Compilation Part 3 50 Minutes of Fun!
Watch Motus love affair with samosas in this amazing compilation now!!!
Click - Like , Share , SUBSCRIBE for more!
Watch more Motu Patlu Videos here:
https://www.youtube.com/wowkidztv
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https://www.facebook.com/WowKidzTV
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https://www.instagram.com/wowkidztv/
Available link for download
Thursday, March 2, 2017
Wednesday, March 1, 2017
Monday Made it 3 For Me!
Monday Made it 3 For Me!
Project #1:
This Monday Made It, is rather disappointing. I had hoped to make my own vinyl. I found a blog on Pinterest that explained how to make homemade vinyl.
http://brandyscrafts.blogspot.com/2010/05/contact-paper-cardstock-wall-decals.html
I thought, that sounds AMAZING! I keep looking at all these different vinyl stores online, but cant make up my mind. Im not a great decision maker when it comes to these sorts of things, and I just flip from shop to shop and never buy a thing. If I could make my own vinyl, having chosen the paper and colors in person well that would solve my problems!
So I tried it. It wasnt easy. Contact paper is a difficult medium! I dont have enough hands to keep it flat seriously I was using my chin at one point. But eventually I was able to sort of work it out, and I anxiously awaited 12 pieces of homemade vinyl.
Well as the cardstock dried it became very loosely stuck to the contact paper. Should I have used more mod podge? Something heavier? I put a heavy binder on them as they dried.
Then, when I tried using my Silhouette on them, it failed. The letters were cut but the cardstock separated from the contact paper. Needless to say Im disappointed and back to square 1 when it comes to vinyl.
Project #2:
I still was able to make my Bienvenidos Banner using my cameo. I just used a touch of hot glue to attach the letters instead of homemade vinyl. The Silhouette cut the pennants (including circles for ribbon) and the letters. It was super easy!
I chose my color scheme with Kristens flipped signs in mind I love everything she does, so this seemed like a safe plan.
Thinking:
Created:
Project #3:
This week I also made these fun duct tape flower pens. (Once I have vinyl I want to add some grass to the tin). I also need to add a bit of foam to the bottom of my recycled Tazo Tea tins, because they are too deep for everything I make. I was thinking they could be some sort of incentive for the kids after so many pieces of bling you get to use the flower pen I could have sold that with second graders, but Im thinking fifth graders wont buy into it theyre still cute though!
I used this tutorial that I found on Pinterest.
It was really easy to follow. I felt like my fingerprints had been ripped off after so much duct tape use, but apparently not since they scanned properly for my background check for my new employer.
Project #4:
I also had ordered these posters from Vistaprint, and had the dry mounted at Hobby Lobby. They were too flimsy and I was afraid they would get destroyed in my classroom. The dry mounting cost $9.00 a piece, but if you consider I got them for free at the clearance sale, I think its a good deal!
Krista @ The Second Grade Super Kids made something earlier this summer with the quote from Gandhi and I loved her idea! Be the change you want to se in the world. These will look great in my classroom and are perfect for dual language!
Find more great ideas at 4th Grade Frolics:

Thats it for this week!
Happy creating!
Mrs. Castro
Available link for download
My Talking Angela v1 8 3 APK DIAMANTES MONEDAS ILIMITADAS
My Talking Angela v1 8 3 APK DIAMANTES MONEDAS ILIMITADAS

Plataforma: Android
Requiere: Android 4.0 o Superior
Tipo: Juegos
Versión: 1.8.3
My Talking Angela apk v1.8.3 hack monedas infinitas apk, baixar My Talking Angela v1.8.3 diamantes ilimitados android & monedas, download My Talking Angela unlimited coins & diamonts free, apkmania My Talking Angela v1.8.3 latest version full apk hack 2015. Bienvenido al mundo glamoroso de My Talking Angela! Adoptar Angela como su propia mascota virtual y darle una vida fabulosa! Es toda tuya para cuidar de gatito a gato.
Descripción
Viste a Angela en la última moda y el vestuario más lindos y darle los peinados más increíbles. Haga su hogar hermoso y alimentar a sus deliciosas golosinas. Sólo ver - se convertirá en su nuevo mejor amigo! Puede jugar juntos, recoger etiquetas engomadas frescas para comerciar con sus amigos, y sólo tienen un montón de diversión. Haga Angela tu propio superestrella!
- Nutre tu ANGELA: Adoptar Angela como un lindo gatito y ayudarla a crecer en un gatito elegante ciudad. A partir de cepillarse los dientes a compras de ropa - ella es todo tuyo!
- Exprese su creatividad: Usted es fabuloso así que Angela también fabuloso! Actualizar y personalizar su casa y un armario en un montón de diferentes maneras. Hacer que se vea exactamente de la manera que quieras!
- VESTIDO JUEGO UP: Deje que su impresionante sentido del estilo brillar a través y vestir a Angela en la última moda y los accesorios más lindos.
- ELEGIR DONDE VIVE: Escoja su lugar favorito para la casa de Angela de un montón de diferentes opciones y personalizar la vista de su increíble terraza.
- JUGAR: Descubre y jugar increíbles nuevos mini juegos!
- Recoger ETIQUETAS: Encuentra y recoge cientos de pegatinas virtuales, cada una decorada con marcas nuevas imágenes de Angela, y colocarlos en su propio álbum súper especial. También puede compartir las pegatinas con otros jugadores para completar su álbum.
- OBTENER LA ROPA EXCLUSIVA: Hacer Angela brillo en trajes sorprendentes recogiendo pegatinas especiales y desbloquear nuevas ropas exclusivas.
- JUEGO CON ANGELA: Angela tiene emociones similares a la vida tan agradable! Engage con ella en la pantalla y ver cómo responde. Usted puede hablar y cantar con ella y ella va a repetir todo!
Esta aplicación está certificada PRIVO. El sello de puerto seguro PRIVO indica Outfit7 ha establecido prácticas de privacidad COPPA compatibles para proteger la información personal de su hijo. Nuestras aplicaciones no permiten niños más pequeños para compartir su información.
Instalar Apk
Copiar carpeta OBB para sdcard / Android / OBB
Iniciar el Juego.
Available link for download
Saturday, February 25, 2017
Friday, February 24, 2017
N O V A 3 Freedom Edition Hack Cheat Tool
N O V A 3 Freedom Edition Hack Cheat Tool
Check N.O.V.A. 3 Freedom Edition Hack Proof and How to use
About N.O.V.A. 3 Freedom Edition Hack Cheat Tool
Hello. You’ve arrived to the right place searching for N.O.V.A. 3 Freedom Edition Hack Cheat Tool. This application is perfect for you to add Unlimited Credits to your account.
You have an Android device where you play? Or maybe an iOS device? Or the game is now available on Facebook platform? N.O.V.A. 3 Freedom Edition Hack Cheat Tool is the right application to add cheats in game.
The great thing about the hack tool is that it does not leave any traces and protects your account from being banned or restricted. Is true that you don’t have to abuse it, but use it wisely.
So when you want to add free Credits to your account use N.O.V.A. 3 Freedom Edition Hack Cheat Tool. It’s free to download right now from Zippyshare or Dropbox and you can enjoy every features starting now.
N.O.V.A. 3 Freedom Edition Hack Cheat Tool Features
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How to use N.O.V.A. 3 Freedom Edition Hack Cheat Tool
1. Download the program from the link bellow
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Screenshots
Statistics
Name: N.O.V.A. 3 Freedom Edition Hack Cheat Tool
Version: Latest
Size: 14.1 MB
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N.O.V.A. 3 Freedom Edition Hack Cheat Tool
Available link for download
Thursday, February 23, 2017
N O V A 3 Near Orbit Vanguard Alliance v1 0 2 Apk
N O V A 3 Near Orbit Vanguard Alliance v1 0 2 Apk

Available link for download
Tuesday, February 21, 2017
More UNCHARTED 3
More UNCHARTED 3






heres a few more concept deigns from Uncharted 3. i was trying out a little more of my graphic look on these.
Available link for download
Monday, February 20, 2017
More Reviews for the 5 3 Comic Week
More Reviews for the 5 3 Comic Week
Marvel Team-Up (Marvel)
Free Scott Pilgrim (Oni)
Available link for download
Thursday, February 16, 2017
Mountain Goat Mountain v1 3 12 Mod
Mountain Goat Mountain v1 3 12 Mod
Requirements: 2.3.3+
Overview: Mountain Goat Mountain is a fast arcade game, where you climb the highest and most treacherous mountain ever seen by a goat.
JUMP up the mountain. Jump up the mountain.. Jump up the mountain you get the idea.
CLIMB to all new heights to show your friends that when the goating gets tough, you get goating.
DODGE lots of things that when falling will most likely squish you. Collect different Goat Hats to travel to different environments, including Arizona Desert, VR Voxels, and Winter Snowland. The game is free to play, however in-app purchases are available for additional content and premium currency. In-app purchases range from $0.99 to $99.99.
Whats New
Bugfixes and Optimizations
This app has Google Mobile Ads advertisements
More Info:
https://play.google.com/store/apps/details?id=com.zynga.mountaingoat
Download: Mod Money
https://userscloud.com/
http://ul.to/
Available link for download
My talking Tom 3 7 1 53 MOD HACK Dinero infinito apk datos
My talking Tom 3 7 1 53 MOD HACK Dinero infinito apk datos
Compatibilidad y más información: Google Play
- Juega a más de 10 minijuegos: ¡Conecta Gominolas, Bubble Shooter, Salto interplanetario y muchos más! ¡Gana monedas de oro y diviértete!
- Graba y mira vídeos: graba y comparte tus propios vídeos de Mi Talking Tom y mira también otros vídeos. (Disponible exclusivamente en determinados dispositivos con Android 4.1 o superior. La lista se puede consultar en http://tinyurl.com/listmtt)
- Cuida de tu propio Tom: juega con él, aliméntalo con sus comidas favoritas, mételo en la cama.
- Visita a tus amigos y los Talking Tom de otros jugadores: ¡echa un vistazo a los apartamentos y aspecto de otros Tom, encuentra cofres del tesoro y consigue monedas!
- Disfruta de emociones reales: Tom puede estar feliz, hambriento, adormilado, aburrido... Sus emociones cambian dependiendo de cómo juegas con él.
- Desata tu creatividad: crea tu propio Tom escogiendo entre 1000 combinaciones de pelaje, ropa y mobiliario.
- Consigue premios a medida que progresas: ¡ayuda a Tom a crecer a lo largo de 9 etapas y 999 niveles desbloqueando nuevos elementos y monedas a medida que avanzas!
- Interactúa con Tom: habla y Tom repetirá todo lo que digas. Empújalo, acarícialo o hazle cosquillas y mira cómo responde.
- Promoción de productos y publicidad contextual de Outfit7
- Enlaces que dirigen a los clientes a nuestros sitios web y otras aplicaciones de Outfit7
- Personalización de contenidos para atraer a los usuarios para que usen de nuevo la aplicación
- La posibilidad de conectarse con amigos a través de redes sociales
- Ver vídeos de personajes animados de Outfit7 mediante la integración con YouTube
- La opción de hacer compras desde la aplicación
- Los artículos están disponibles para diferentes precios en moneda virtual, dependiendo del nivel actual alcanzado por el jugador
Available link for download
Wednesday, February 15, 2017
MY TALKING TOM V 3 8 1 57 APK MOD INFINITE MONEY
MY TALKING TOM V 3 8 1 57 APK MOD INFINITE MONEY

- Bermain lebih dari 10 mini-games: Memory Game, Whack-A-Mouse, Connect dan banyak lagi! Mendapatkan koin emas dan bersenang-senang!
- Rekam & menonton video: Merekam dan berbagi sendiri video My Talking Tom dan menonton video lain juga. (Hanya tersedia pada perangkat pilih menjalankan Android 4.1+
- Memelihara sendiri Tom Anda: Bermain game dengan dia, memberinya makan makanan favoritnya, menyelipkan dirinya ke tempat tidur.
- Kunjungi teman & pemain lain Talking Toms: Check out apartemen dan terlihat dari Toms lainnya, menemukan peti harta karun dan mendapatkan uang!
- Nikmati emosi hidup seperti: Tom bisa bahagia, lapar, mengantuk, bosan ... emosinya berubah sesuai dengan bagaimana Anda bermain dengan dia.
- Melepaskan kreativitas Anda: Buat Anda Tom sendiri dengan memilih dari 1000 dari kombinasi bulu, pakaian dan furniture.
- Mendapatkan hadiah karena Anda maju: Bantuan Tom tumbuh melalui 9 tahapan yang berbeda dan 999 tingkat membuka item baru dan koin yang Anda pergi!
- Berinteraksi dengan Tom: Talk dan Tom masih mengulangi semua yang Anda katakan. Poke, stroke dan menggelitik dia, dan melihat bagaimana dia merespon.
- Infinite Money
Available link for download
Monday, February 13, 2017
My Talking Tom 3 7 6 97 Mod Apk
My Talking Tom 3 7 6 97 Mod Apk

Descubre el # 1 juegos de aplicación en 135 países! Adoptar su propio gatito del bebé y ayudarle a crecer en un gato completamente crecido. Tome el buen cuidado de su mascota virtual, nombrarlo y le hará parte de su vida diaria dándole de comer, jugar con él y nutrir a medida que crece.
vestirlo como quieras y elegir entre una amplia selección de colores de piel y otros accesorios. Decorar su casa y ver cómo otros decoraban sus hogares Mi Talking Tom. Jugar con su Tom y ver como se convierte en una parte de su vida cotidiana.
QUÉ HAY DE NUEVO:
Viajar por el mundo: Ver y conocer a otros Toms de todo el mundo!
Trendsetter: 3 a estrenar trajes para Tom ponerse!
MEJORAS: Véase el nuevo vestidor de Tom!
Más logros: 4 nuevos logros para ganar!
Como Instalar:
"APK" instalarlo en su dispositivo.
Entrar en el juego.
Configuración: Mod
DESCARGAR APK MOD
Available link for download
Sunday, February 12, 2017
My Me Made May16 Week 3
My Me Made May16 Week 3
Week THREE?! How did that happen? Does anyone else feel this month is flying by in a flash? Ive barely had a chance to snoop through the Flickr group or through the #MMMay16 hashtag on IG. Anyways, at least both of those will remain as a permanent record of the challenge to be enjoyed at a later date when (if) life calms down. On with the documentation...








Available link for download
Saturday, February 11, 2017
Molecular Evidence 3 Transposons
Molecular Evidence 3 Transposons
All right, this is the third podcast in a series of six that Ive planned on the molecular evidence for evolution. Ill be using Dr. Douglas Theobalds resource on Talk.Origins.org pretty heavily, so you can follow along with me there if you like.
The third piece of evidence is from transposons.
Now, youll remember that back in episode 106, we looked at junk DNA, and what it meant. Well, for the past two weeks weve been looking at the coding part of the genome, essentially the genes themselves and the products of their transcription, that is, proteins. For the next three weeks were going to leave those evidences behind in the coding part of the genome, and were going to look at the noncoding part, which some people call junk. Thats right- one mans junk is another mans treasure, and so-called junk DNA is a treasure of evidence, very powerful evidence in support of evolutionary theory.
Well, what is a transposon? A transposon is a mobile section of DNA. What I mean by saying that its mobile is that it can literally change its position within the genome. Im afraid that there really arent any good analogies for this, so Ill just have to resort to some bad ones. You remember that before I said that the genome is kinda like a magazine, where the genes are articles which are separated by pages and pages of advertisements. Well, you know those annoying advertisements that, instead of being printed in the pagestock, are printed on little cards and just kinda stuck in the magazine, near the spine? Or maybe one side of the card is glued to the page with that flimsy, rubbery glue that you have to peel and pull off at the same time? Thats basically what a transposon is. In the same way that those little cards are mobile advertisements, a transposon is mobile DNA.
Lets say that youve got one of those annoying sticky cards in your magazine, and you pull it out. But then you accidentally drop both the card and the magazine, and they both land together. The card is going to be restuck in the magazine, but probably not in the same place. It might be stuck to the front cover. It might be stuck on another advertisement page that had nothing to do with where it came from. Or, it might be stuck on the story that you were reading, obstructing a couple paragraphs and preventing you from finishing the article. Well, thats also what happens with transposons. A transposon can be cut out of the genome and then reinserted someplace else. The genome is a pretty big sequence, so theres lots of places a transposon can reinsert. Sometimes a transposon will reinsert at another noncoding region. Actually, this is usually what happens, since theres so much more noncoding DNA than there is coding DNA. But sometimes a transposon can reinsert in a coding region, and disrupt a gene. Now, in the same way that the stuck card in your magazine prevents you from reading the story, the inserted transposon prevents the gene from being transcribed, effectively turning it off. You might also think of a transposon like a pop-up ad on a website that pops up out of nowhere and obscures the content that youre trying to see on the page.
Now, one of the obvious questions at this point is: why in the world do transposons exist? They seem pretty annoying, from a strictly genetic perspective, and they also seem dangerous, since by inserting into a gene a transposon could cause a debilitating mutation or disease. And indeed, this is the case- transposons are mutagenic, and are associated with a number of diseases, including hemophilia, severe immunodeficiency, and cancer. So why do transposons exist in genomes at all? Well, you can think of a transposon as existing as a separate selective entity to its host genome- almost like a DNA parasite. Now, admittedly, this is a hard concept to grasp, since were talking about a chunk of DNA and not something typically associated the word parasite, like a mosquito or a tick. But remember that even though its easier for us to think of concepts in black and white terms, science isnt quite so discrete. Transposons come in two basic types: class I transposons, which are also called retrotransposons, and class II, which are simply DNA transposons. Retrotransposons function by allowing their sequence to be transcribed into RNA. Its at this point that a retrotransposon does something odd- it reverse-transcribes the RNA sequence back into DNA, and this DNA copy of the original retrotransposon sequence is then integrated back into the original genome, but at a different location. Both of these functions are carried out by enzymes whose genes are encoded for within the retrotransposon sequence itself- pretty clever. In fact, this is the same way that retroviruses like HIV work- except that a retrotransposon never leaves the cell in a virus particle. You can almost think of a retrotransposon as a virus that made itself comfortable within an organism and decided never to leave. DNA transposons use a different enzyme called transposase, which actually cuts out the genomic transposon sequence and puts it back into the genome in a different location. This skips the whole process of reverse transcription of RNA that retrotransposons use, but you get the same basic effect.
Retrotransposons themselves come in two basic types- long and short. The longer ones are called long interspersed elements, or LINEs. The shorter ones are called short interspersed elements, or SINEs. LINEs contain the two enzymes necessary for the reverse transcription and integration that I already mentioned- called, predictably, reverse transcriptase and integrase. SINEs, on the other hand, dont carry these genes, and so are dependent on LINEs for their propagation. You can think of the enzymes used by the retrotransposons as a copy and paste function, just like in a word processor. The transposase used by the DNA transposons is more like the cut and paste function, however. And Im sure you know that if you cut and paste words in a document, you may screw up the meaning of the text, but youre not going to significantly add or subtract to the length of the text. If you copy and paste, though, youll find that not only have you screwed up the meaning of the text, but youve also added overall length to it, and depending on how many times you paste, you may have added a lot of length to it. And thats what we see with retrotransposons- both LINEs and SINEs are found all throughout the human genome, for example, and are responsible for nearly 30% of the total size of the genome. 30%! Thats a lot of space wasted on DNA parasites.
But its not all for naught. Because so much of the genome is made up of these predictable sequences, and because these sequences occur randomly in different places in the genome, transposons offer an excellent way to identify individuals genetically. Im sure youve heard of technology like DNA fingerprinting, or something similar, that is used to establish paternity using a genetic test. These tests take advantage of the fact that two different individuals in a population having the transposon sequences in the exact same location is extremely rare, so much so that you can conclude genetic relation based on similar patterns of genomic transposons. Well, Im sure youre all astute enough to realize that if transposons can be used to establish a hereditary relationship between a father and his offspring, it can also be used to establish a hereditary relationship between two organisms from different species! Remember, the only observed mechanism for two organisms to have similar genomic sequence is through heredity, and so if two different species can be shown to have similar genomic sequences, then we can conclude that they share a common ancestry. So we hypothesize that if evolutionary theory is correct, and different species share common ancestry, then closely related species will share common transposon insertions. So lets look at the evidence.
Well look at one of the common SINE retrotransposons, called the Alu element. This is a sequence only about 300 nucleotides long, and it found in all mammal species, and particularly in humans, where it composes close to 10% of the entire genome. In alpha-globin gene cluster, 7 separate Alu elements are known to exist, and all seven are found in the exact same location in the corresponding chimpanzee gene. According to our hypothesis, corresponding transposon sequences imply shared ancestry, and thus this evidence supports evolutionary theory.
So, to review, transposons are mobile DNA sequences that create distinct insertion patterns that allow us to distinguish hereditary links between individuals of the same species, as well as to establish common ancestry between organisms of different species. Once again, the evidence of common transposon insertions in humans and chimpanzees strongly supports the evolutionary hypothesis. Next week, well look at pseudogenes, and how these broken genes also support evolutionary theory. Take care!
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